CD Genomics is promoting its RNA Methylation Sequencing Service for research teams investigating chemical modifications that influence RNA behavior after transcription. The service supports the study of m6A, m1A, m5C, m7G, ac4C, 2′-O-methylation and pseudouridine across messenger RNA and noncoding RNA species.
RNA modifications can affect transcript stability, structure, processing, localization and translation. As epitranscriptomics research expands beyond a single modification, method selection becomes an important part of experimental design. Different modification classes may require antibody-based enrichment, bisulfite conversion, immunoprecipitation, direct long-read sequencing or other specialized workflows.
CD Genomics provides RNA modification profiling across coding and noncoding transcripts through a portfolio that includes m6A RNA methylation sequencing, m5C RNA-BS-seq, RIP-seq, Nanopore RNA methylation sequencing, 2′-O-methylated-seq, acRIP-seq and PA-Ψ-seq. These approaches can be selected according to the modification of interest, RNA species, expected signal and desired resolution.
For m6A studies, MeRIP-based sequencing uses modification-specific antibodies to enrich RNA fragments before sequencing. This approach can support transcriptome-wide analysis of m6A-associated regions in mRNA, long noncoding RNA and circular RNA. For m5C studies, RNA bisulfite sequencing converts unmodified cytosines while retaining methylated cytosines, allowing researchers to assess m5C-associated sequence changes after sequencing and computational analysis.
Other workflows address different analytical questions. Nanopore-based RNA methylation sequencing can provide direct long-read information for multiple modification types, while 2′-O-methylated-seq focuses on ribose methylation patterns across RNA molecules. acRIP-seq is designed to profile acetylated RNA regions, and PA-Ψ-seq supports mapping of pseudouridine sites with high-resolution sequencing strategies.
Potential research applications include studies of post-transcriptional gene regulation, RNA stability, translation control, alternative splicing, RNA processing, ribosome biogenesis and the relationships between RNA modifications and cellular responses. The service may also support projects involving mRNA, lncRNA, circRNA, tRNA, rRNA and other RNA classes, depending on the selected protocol.
Interpretation should remain specific to the assay used. Antibody-enrichment methods provide modification-associated regions rather than necessarily identifying a modification at single-nucleotide resolution. Bisulfite-based methods require careful attention to conversion efficiency, RNA structure and sequence context, while direct sequencing approaches depend on platform performance and computational interpretation. Detection of a modified site alone does not establish its functional or causal effect.
About CD Genomics:
CD Genomics provides sequencing and bioinformatics support for epitranscriptomics and other genomics research applications. Its portfolio includes next-generation sequencing, transcriptomics, epigenomics, genotyping, long-read sequencing and customized data-analysis services.
Media Contact:
Contact Person: Dianna Gellar
Email: contact@cd-genomics.com
Phone: +1 631 259 7705
Website: cd-genomics.com
Address: Shirley, NY, USA
For research purposes only, not intended for clinical diagnosis, treatment, or individual health assessments.